Review



pet30a alkb wt  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Addgene inc pet30a alkb wt
    Pet30a Alkb Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet30a+alkb+wt/pET30a-AlkB+(Plasmid+%2379050)/pmc11233606-290-0-1
    Average 93 stars, based on 15 article reviews
    pet30a alkb wt - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Plasmid Preparation:

    Article Title: Nucleotide resolution profiling of m 3 C RNA modification by HAC-seq
    Article Snippet: For LC/MS-MS data collection and analysis, Agilent Mass Hunter LC/MS Data Acquisition Version B.08.00 and Quantitative Analysis Version B.07.01 software was used. .. pET30a-AlkB-WT and pET30a-AlkB-D135S plasmids from Tao Pan (Addgene plasmid # 79050 and #79051) were transfected into One Shot ® BL21 StarTM (DE3) competent cells. ..

    Article Title: ALL-tRNAseq enables robust tRNA profiling in tissue samples.
    Article Snippet: Quality and integrity of all RNA samples were measured on a NanodropOne (Thermo Fisher Scientific) and Agilent 2100 bioanalyzer (Agilent Technologies) using an RNA 6000 Nano kit (Agilent Technologies 5067-1511). .. Expression and purification of AlkB WT and mutant enzymes Expression and purificationmethodswere adapted frompreviously published methods (Cozen et al. 2015; Zheng et al. 2015; Hrabeta-Robinson et al. 2017). pET30a-AlkB-WT and pET30a-AlkBD135S, a truncated AlkB with deletion of the N-terminal 11 GENES & DEVELOPMENT 253 amino acids, were a gift from Tao Pan (Addgene plasmid #79050 [http://n2t.net/addgene:79050; RRID: Addgene_79050] and #79051 [http://n2t.net/addgene:79051; RRID: Addgene_79051], respectively). ..

    Transfection:

    Article Title: Nucleotide resolution profiling of m 3 C RNA modification by HAC-seq
    Article Snippet: For LC/MS-MS data collection and analysis, Agilent Mass Hunter LC/MS Data Acquisition Version B.08.00 and Quantitative Analysis Version B.07.01 software was used. .. pET30a-AlkB-WT and pET30a-AlkB-D135S plasmids from Tao Pan (Addgene plasmid # 79050 and #79051) were transfected into One Shot ® BL21 StarTM (DE3) competent cells. ..

    Article Title: m 3 C32 tRNA modification controls serine codon-biased mRNA translation, cell cycle, and DNA-damage response
    Article Snippet: .. pET30a-AlkB-WT (Addgene, #79050) and pET30a-AlkB-D135S (Addgene, #79051) plasmids were transfected into One Shot® BL21 Star TM (DE3) chemically competent cells (Invitrogen, #C601003). ..

    Expressing:

    Article Title: ALL-tRNAseq enables robust tRNA profiling in tissue samples.
    Article Snippet: Quality and integrity of all RNA samples were measured on a NanodropOne (Thermo Fisher Scientific) and Agilent 2100 bioanalyzer (Agilent Technologies) using an RNA 6000 Nano kit (Agilent Technologies 5067-1511). .. Expression and purification of AlkB WT and mutant enzymes Expression and purificationmethodswere adapted frompreviously published methods (Cozen et al. 2015; Zheng et al. 2015; Hrabeta-Robinson et al. 2017). pET30a-AlkB-WT and pET30a-AlkBD135S, a truncated AlkB with deletion of the N-terminal 11 GENES & DEVELOPMENT 253 amino acids, were a gift from Tao Pan (Addgene plasmid #79050 [http://n2t.net/addgene:79050; RRID: Addgene_79050] and #79051 [http://n2t.net/addgene:79051; RRID: Addgene_79051], respectively). ..

    Purification:

    Article Title: ALL-tRNAseq enables robust tRNA profiling in tissue samples.
    Article Snippet: Quality and integrity of all RNA samples were measured on a NanodropOne (Thermo Fisher Scientific) and Agilent 2100 bioanalyzer (Agilent Technologies) using an RNA 6000 Nano kit (Agilent Technologies 5067-1511). .. Expression and purification of AlkB WT and mutant enzymes Expression and purificationmethodswere adapted frompreviously published methods (Cozen et al. 2015; Zheng et al. 2015; Hrabeta-Robinson et al. 2017). pET30a-AlkB-WT and pET30a-AlkBD135S, a truncated AlkB with deletion of the N-terminal 11 GENES & DEVELOPMENT 253 amino acids, were a gift from Tao Pan (Addgene plasmid #79050 [http://n2t.net/addgene:79050; RRID: Addgene_79050] and #79051 [http://n2t.net/addgene:79051; RRID: Addgene_79051], respectively). ..

    Mutagenesis:

    Article Title: ALL-tRNAseq enables robust tRNA profiling in tissue samples.
    Article Snippet: Quality and integrity of all RNA samples were measured on a NanodropOne (Thermo Fisher Scientific) and Agilent 2100 bioanalyzer (Agilent Technologies) using an RNA 6000 Nano kit (Agilent Technologies 5067-1511). .. Expression and purification of AlkB WT and mutant enzymes Expression and purificationmethodswere adapted frompreviously published methods (Cozen et al. 2015; Zheng et al. 2015; Hrabeta-Robinson et al. 2017). pET30a-AlkB-WT and pET30a-AlkBD135S, a truncated AlkB with deletion of the N-terminal 11 GENES & DEVELOPMENT 253 amino acids, were a gift from Tao Pan (Addgene plasmid #79050 [http://n2t.net/addgene:79050; RRID: Addgene_79050] and #79051 [http://n2t.net/addgene:79051; RRID: Addgene_79051], respectively). ..



    Similar Products

    93
    Addgene inc pet30a alkb wt
    Pet30a Alkb Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet30a+alkb+wt/pET30a-AlkB+(Plasmid+%2379050)/pmc11233606-290-0-1
    Average 93 stars, based on 1 article reviews
    pet30a alkb wt - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Addgene inc recombinant alkb purification pet30a alkb wt
    Recombinant Alkb Purification Pet30a Alkb Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet30a+alkb+wt/pET30a-AlkB+(Plasmid+%2379050)/pm38982125-292-0-4
    Average 93 stars, based on 1 article reviews
    recombinant alkb purification pet30a alkb wt - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    Image Search Results